genotoxic damage to glioblastoma cells treated with 6 mv x-radiation in the presence or absence of methoxy estradiol, iudr or topotecan

نویسندگان

nazila eyvazzadeh

ali neshasteh-riz

seyed rabee mahdavi

afshin mohsenifar

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Genotoxic Damage to Glioblastoma Cells Treated with 6 MV X-Radiation in The Presence or Absence of Methoxy Estradiol, IUDR or Topotecan

OBJECTIVE To explore the cumulative genotoxic damage to glioblastoma (GBM) cells, grown as multicellular spheroids, following exposure to 6 MV X-rays (2 Gy, 22 Gy) with or without, 2- methoxy estradiol (2ME2), iododeoxyuridine (IUDR) or topotecan (TPT), using the Picogreen assay. MATERIALS AND METHODS The U87MG cells cultured as spheroids were treated with 6 MV X-ray using linear accelerator....

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dna damage of glioblastoma multiform cells induced by beta radiation of iodine-131 in the presence or absence of topotecan: a picogreen and colonogenic assay

objective: glioblastoma multiforme (gbm), one of the most common and aggressive malignant brain tumors, is highly resistant to radiotherapy. numerous approaches have been pursued to find new radiosensitizers. we used a picogreen and colonogenic assay to appraise the dna damage and cell death in a spheroid culture of gbm cells caused by iodine-131 (i-131) beta radiation in the presence of topote...

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DNA Damage of Glioblastoma Multiform Cells Induced by Beta Radiation of Iodine-131 in The Presence or Absence of Topotecan: A Picogreen and Colonogenic Assay

OBJECTIVE Glioblastoma multiforme (GBM), one of the most common and aggressive malignant brain tumors, is highly resistant to radiotherapy. Numerous approaches have been pursued to find new radiosensitizers. We used a picogreen and colonogenic assay to appraise the DNA damage and cell death in a spheroid culture of GBM cells caused by iodine-131 (I-131) beta radiation in the presence of topotec...

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Hirschsprung Disease Diagnosis: Calretinin Marker Role in Determining the Presence or Absence of Ganglion Cells

Background: Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections fr...

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McDonaldization in Iranian ELT Textbooks: Absence or Presence?

The development of materials for language teaching has been retraced from different perspectives. For example, some have identified influences of a social view on designing course books and compiling materials for language classes. The purpose of this article is to focus on the specific case of ELT material, its design and priorities in the context of Iran. This article also offers a detailed a...

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Sperm DNA damage in mice irradiated with various doses of X-rays alone or in combination with actinomycin D or bleomycin sulfate: an in vivo study

Background: DNA damage in male germ cells due to exposure to environmental and manmade physico-chemical genotoxic agents is considered as the main cause of male infertility. The aim of this study was to evaluate the effects of combined modalities (radiotherapy and chemotherapy) routinely used for cancer treatment on mouse sperm chromatin in vivo. Materials and Methods: Forty-eight mice were div...

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عنوان ژورنال:
cell journal

جلد ۱۷، شماره ۲، صفحات ۳۱۲-۳۲۱

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